Free Transposon Vector Plasmid – TnCarrier-1

Get your free transposon vector plasmid today!
TnCarrier-1 is a compact, high-performance plasmid backbone designed for easy creation of custom transposons. This vector combines efficient transposon isolation, simple modification, and a γ-origin that prevents any experimental background — and we provide it free of charge for research use.

Why Choose TnCarrier-1?

1. Easy customization
You can easily insert your own cargo construct into Tn-Carrier1 or use PCR to introduce custom-designed modifications, e.g. restriction sites, at the transposon ends. All this provides maximum flexibility in the customization of your transposon of choice.

2. Small vector backbone allows highly efficient transposon isolation
The vector backbone moiety is significantly shorter than customary transposons, allowing straightforward and effective transposon purification due to the large size difference.

3. Background-free experiments
The plasmid with the γ-origin of replication requires a PIR1 E. coli strain for propagation. This ensures that any potential background from the original plasmid circles is eliminated when standard E. coli hosts are used in Mu transposition experiments.