A comprehensive genome-wide insertion mutant library for archaeal functional genomics.
Haloferax volcanii insertion library
The Haloferax volcanii insertion library is a comprehensive genome-wide collection of random transposon insertion mutants covering all non-essential genes of the model archaeon Haloferax volcanii. The library enables rapid gene discovery, phenotype screening, functional genomics, and archaeal genetics without the need to construct a mutant collection from the scratch.
The library consists of Haloferax volcanii H295 mutants, each carrying a single TrpA-cat-Mu transposon inserted at a random chromosomal location, providing complete genome-wide coverage (1). The transposon contains the trpA selectable marker for H. volcanii and the cat selectable marker for Escherichia coli.
Why choose this library?
Genome-Wide Coverage
Covers all non-essential genes.
Research-Proven
Validated in multiple peer-reviewed studies (1-5).
Ready-to-Use
Delivered as convenient aliquots.
Functional Genomics
Ideal for phenotype screening and gene discovery.
Model Archaeon
Built using the leading archaeal model organism.
Single-Insertion Mutants
Reliable genotype–phenotype analysis.
COMPLETE GENOME-WIDE LIBRARY — 30% OFF
~28,000 insertion mutants
2,500 €
1,750 €
Use code: HALOFERAX2026
Offer valid through December 31, 2026.
| Catalog Number | Units | Volume | Price (VAT 0%) | |
| D030104 | approx. 1,000 clones | 1×100 µl | 350 € | Buy now |
| D030103 | approx. 5,000 clones | 5×100 µl | 700 € | Buy now |
| D030102 | approx. 10,000 clones | 10×100 µl | 1,200 € | Buy now |
| ⭐D030101 | approx. 28,000 clones | 28×100 µl | Buy now |
⭐ Special Offer: Complete Genome-Wide Library now available for 1,750 € (30% off)
Use code: HALOFERAX2026
Offer valid through December 31, 2026.
Applications
- Functional genomics
- Gene discovery
- Reverse genetics
- Phenotype screening
- Archaeal cell biology
- Motility studies
- Biofilm formation
- Stress response
- Metabolism
- Membrane biology
PhD Elsi Pulkkinen
Senior Scientist
Domus Biotechnologies
Talk with a scientist
We’re happy to discuss your research goals and help you determine whether the Haloferax volcanii insertion mutant library is suitable for your project. Our team can provide guidance on experimental design, mutant screening strategies, functional genomics applications, and the effective use of the library for gene discovery and archaeal biology research.
References:
- Kiljunen, S., Pajunen, M.I., Dilks, K., Storf, S., Pohlschroder, M. and Savilahti, H. 2014: Generation of comprehensive transposon insertion mutant library for the model archaeon, Haloferax volcanii, and its use for gene discovery. – BMC Biology 12: 103.
- Legerme, G., Yang, E., Esquivel, R.N., Kiljunen, S., Savilahti, H. and Pohlschroder, M. 2016: Screening of a Haloferax volcanii transposon library reveals novel motility and adhesion mutants. – Life 6: 41.
- Gomez, M., Leung, W., Dantuluri, S., Pillai, A., Gani, Z., Hwang, S., Lana, J., McMillan, L.J., Kiljunen, S., Savilahti, H. and Maupin-Furlow, J.A. 2018: Molecular factors of hypochlorite tolerance in the hypersaline archaeon Haloferax volcanii. – Genes 9: 562.
- Cerletti, M., Rabino, A., Paggi, R.A., Ferrari, C., Poetsch, A., Savilahti, H., Kiljunen, S. and De Castro, R.E. 2022: The C-terminal region of phytoene synthase is a key element to control carotenoid biosynthesis in the haloarchaeon Haloferax volcanii. – Biochemical Journal 479: 2365–2377.
- Egan, M.S., Hogan, K., Maupin-Furlow, J., Pohlschroder, M. 2022: The best of both worlds: discovery-driven learning through a lab-seminar approach. – J. Microbiol. Biol. Educ. 23:e00031-22.
For Research Use Only. Not for use in diagnostic procedures.