Our Haloferax volcanii insertion mutant library contains an exhaustive pool of mutants, in which each clone contains one genomic insertion of a transposon in a random position and with a full-genome coverage.
Insertion mutant libraries
Want to make your own insertion mutant library?
Below you can find recommended products for the generation of your own insertion mutant library
| Organism | Transposase | Transposon | Reference |
| Gram-negative bacteria | v.3 MuA Transposase (in vivo Integrator) | Cat-Mu Transposon (CamR) | 1 |
| Gram-negative bacteria | v.3 MuA Transposase (in vivo Integrator) | Kan-Mu Transposon (KanR) | 1 |
| Gram-positive bacteria | v.3 MuA Transposase (in vivo Integrator) | Em-Mu Transposon (EmR) | 2 |
Have questions? We are happy to help you!
Contact us
Citations and references
1. Lamberg A, Nieminen S, Qiao M, Savilahti H (2002) Efficient insertion mutagenesis strategy for bacterial genomes involving electroporation of in vitro-assembled DNA transposition complexes of bacteriophage Mu. Appl Environ Microbiol 68:705-712
2. Pajunen MI, Pulliainen AT, Finne J, Savilahti H (2005) Generation of transposon insertion mutant libraries for Gram-positive bacteria by electroporation of phage Mu DNA transposition complexes. Microbiology 151:1209-1218
3. Paatero AO, Turakainen H, Happonen LJ, Olsson C, Palomäki T, Pajunen MI, Meng X, Otonkoski T, Tuuri T, Berry C, Malani N, Frilander MJ, Bushman FD, Savilahti H (2008) Bacteriophage Mu integration in yeast and mammalian genomes. Nucleic Acids Res 36:e148
4. Tu Quoc PH, Genevaux P, Pajunen M, Savilahti H, Georgopoulos C, Schrenzel J, Kelley WL (2007) Isolation and characterization of biofilm formation-defective mutants of Staphylococcus aureus. Infect Immun 75:1079-1088
5. Wu Z, Xuanyuan Z, Li R, Jiang D, Li C, Xu H, Bai Y, Zhang X, Turakainen H, Saris PE, Savilahti H, Qiao M (2009) Mu transposition complex mutagenesis in Lactococcus lactis-identification of genes affecting nisin production. J Appl Microbiol 106:41-8
6. Kiljunen S, Pajunen MI, Dilks K, Storf S, Pohlschroder M, Savilahti H (2014) Generation of comprehensive transposon insertion mutant library for the model archaeon, Haloferax volcanii , and its use for gene discovery. BMC Biol 12:103