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Insertion mutant libraries

Haloferax volcanii insertion mutant library


Our Haloferax volcanii insertion mutant library contains an exhaustive pool of mutants, in which each clone contains one genomic insertion of a transposon in a random position and with a full-genome coverage.


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Want to make your own insertion mutant library?

Transposons are powerful tools for the generation of insertion mutant libraries in various organisms, in which each gene is tagged by an insertion.

Below you can find recommended products for the generation of your own insertion mutant library

Organism Transposase Transposon Reference
Gram-negative bacteria v.3 MuA Transposase (in vivo Integrator) Cat-Mu Transposon (CamR) 1 
Gram-negative bacteria v.3 MuA Transposase (in vivo Integrator) Kan-Mu Transposon (KanR) 1 
Gram-positive bacteria v.3 MuA Transposase (in vivo Integrator) Em-Mu Transposon (EmR) 2


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Citations and references

1. Lamberg A, Nieminen S, Qiao M, Savilahti H (2002) Efficient insertion mutagenesis strategy for bacterial genomes involving electroporation of in vitro-assembled DNA transposition complexes of bacteriophage Mu. Appl Environ Microbiol 68:705-712
2. Pajunen MI, Pulliainen AT, Finne J, Savilahti H (2005) Generation of transposon insertion mutant libraries for Gram-positive bacteria by electroporation of phage Mu DNA transposition complexes. Microbiology 151:1209-1218
3. Paatero AO, Turakainen H, Happonen LJ, Olsson C, Palomäki T, Pajunen MI, Meng X, Otonkoski T, Tuuri T, Berry C, Malani N, Frilander MJ, Bushman FD, Savilahti H (2008) Bacteriophage Mu integration in yeast and mammalian genomes. Nucleic Acids Res 36:e148
4. Tu Quoc PH, Genevaux P, Pajunen M, Savilahti H, Georgopoulos C, Schrenzel J, Kelley WL (2007) Isolation and characterization of biofilm formation-defective mutants of Staphylococcus aureus. Infect Immun 75:1079-1088
5. Wu Z, Xuanyuan Z, Li R, Jiang D, Li C, Xu H, Bai Y, Zhang X, Turakainen H, Saris PE, Savilahti H, Qiao M (2009) Mu transposition complex mutagenesis in Lactococcus lactis-identification of genes affecting nisin production. J Appl Microbiol 106:41-8
6. Kiljunen S, Pajunen MI, Dilks K, Storf S, Pohlschroder M, Savilahti H (2014) Generation of comprehensive transposon insertion mutant library for the model archaeon, Haloferax volcanii , and its use for gene discovery. BMC Biol 12:103