We are here to help you find the right tools for your transposon mutagenesis application, whether it is gene delivery, insertional mutagenesis, knocking out genes, functional and structural studies of proteins, or something else. There are numerous ways how transposon technology can be used in genetic research and the number of applications keeps growing!
OUR PRODUCTS
We Offer Hyperactive MuA Transposases, Transposons, Kits and Insertion Mutant Libraries
MuA Transposases
Domus Biotechnologies provides MuA transposases for in vitro and in vivo transposon mutagenesis.
Our highly efficient MuA variants (v.2 MuA Transposase and v.3 MuA Transposase) are excellent partners for demanding molecular biology applications, both for bacteria and eukaryotic cells.
Our MuA transposases can be used to insert any artificial transposon with MuA binding sites at both ends, into virtually any target DNA in random sites.
Transposons
Domus Biotechnologies provides a wide selection of ready-to-use linear transposons for transposon mutagenesis, covering selectable markers for gram-negative bacteria, gram-positive bacteria and eukaryotic cells.
Genomic Integration Kit
Genomic Integration Kit (CamR) has been designed for efficient mutagenesis of all types of gram-negative bacterial strains.
Genomic Integration Kit is easy to use, simply electroporate Mu DNA Transposition Complexes (CamR) (transpososome) into your bacterial strain of interest. To select the clones with transposon integration, grow bacterial clones on selective (chloramphenicol) media.
Electroporation of Mu DNA transposition complexes has been used successfully with many bacterial species, including gram-negatives, such as coli, Salmonella enterica, Erwinia carotovora, Yersinia enterocolitica, Erwinia carotovora, Pseudomonas aeruginosa.
Haloferax volcanii
insertion mutant library
Transposons are powerful tools for the generation of insertion mutant libraries in various organisms, in which each gene is tagged by an insertion.
Our Haloferax volcanii library contains an exhaustive pool of mutants, in which each clone contains one genomic insertion of a transposon in a random position and with a full-genome coverage.
TRANSPOSON MUTAGENESIS
Transposable elements, or transposons, are DNA segments that can move within a genome, making them powerful tools for studying and manipulating genes across a wide range of organisms, including prokaryotes, plants, invertebrates, and vertebrates. Transposons are versatile because the DNA between their ends can include features like selectable markers, reporter genes, and regulatory elements. Widely used transposon systems include Sleeping Beauty, Tol2, PiggyBac, Tn5, and Mu, with factors like transposase-to-transposon ratio, DNA form, and cell type affecting their efficiency.
Why are Mu transposons ideal for genetic research?
The minimal Mu transposition system is characterized by high transposition frequency and low target-site selectivity, which makes it ideal for applications such as DNA sequencing, protein engineering, functional genome mapping, gene targeting, insertional mutagenesis, and SNP discovery.
Did you know Mu transposons can also deliver genes to mammalian cells?
Mu transposons have demonstrated efficient, species-independent gene delivery and insertional mutagenesis in a range of organisms, including bacteria, yeast, and mammalian cells. While PiggyBac, Sleeping Beauty, and Tol2 are widely used for gene delivery in mammalian cells, a notable advantage of Mu is its use of protein-DNA complexes for gene delivery. In contrast, transposon systems like PiggyBac, Sleeping Beauty, and Tol2 generally depend on plasmid-based methods for transposon delivery and integration. Read more about the current applications for Mu in vitro transposition technology here.
Free consultation available!
We are here to help you to design your experiments and find the right tools for your transposon mutagenesis application!
Contact us
Recent Publications
A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction
MuA Transposase
Protein engineering
Synthetic biology
Mu transpososome activity-profiling yields hyperactive MuA variants for highly efficient genetic and genome engineering
Hyperactive MuA Transposase
Cat-Mu Transposon
Genetic engineering
Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants and genome engineering
MuA Transposase
Haloferax volcanii
Insertion mutant library
Accessible and Insightful Scientific Learning Experiences Using the Microorganism Haloferax volcanii
MuA Transposase
Haloferax volcanii insertion mutant library
Archaea
Education
High school students