Innovative Genome Engineering Solutions for Research and Biotechnology
At Domus Biotechnologies, we provide cutting-edge tools and services for genome engineering, mutagenesis, and protein engineering. Our portfolio includes genomic integration kits, hyper-active MuA Transposases, and customizable transposon design, and purification services, enabling seamless workflows for genome engineering in bacteria, yeast, and mammalian systems. By combining innovative products with expert technical support, we help researchers and biotech teams accelerate discovery and build powerful genetic tools faster.
OUR PRODUCTS
We Offer Genomic Integration Kits, Hyperactive MuA Transposases, Transposons, and Insertion Mutant Libraries
Genomic Integration Kits
Our Genomic Integration Kits provide a powerful, ready-to-use solution for stable genomic integration and mutagenesis across a wide range of hosts, including Gram-negative and Gram-positive bacteria, yeast, and mammalian cells. Each kit includes pre-assembled Mu DNA transposition complexes (transpososomes) with selectable markers such as chloramphenicol (CamR), kanamycin (KanR), or fluorescent reporters, giving you flexible options for different research workflows.
MuA Transposases
Domus Biotechnologies provides MuA transposases for in vitro and in vivo transposon mutagenesis.
Our highly efficient MuA variants (v.2 MuA Transposase and v.3 MuA Transposase) are excellent partners for demanding molecular biology applications, both for bacteria and eukaryotic cells.
Our MuA transposases can be used to insert any artificial transposon with MuA binding sites at both ends, into virtually any target DNA in random sites.
Transposons
Domus Biotechnologies provides a wide selection of ready-to-use linear transposons for transposon mutagenesis, covering selectable markers for gram-negative bacteria, gram-positive bacteria and eukaryotic cells.
Haloferax volcanii
insertion mutant library
Transposons are powerful tools for the generation of insertion mutant libraries in various organisms, in which each gene is tagged by an insertion.
Our Haloferax volcanii library contains an exhaustive pool of mutants, in which each clone contains one genomic insertion of a transposon in a random position and with a full-genome coverage.
OUR SERVICES
We Offer Customizable Transposon Design, and Purification Services
Transposon Design & Generation
Work with our experts to design a custom transposon tailored to your research goals. We can also generate the transposon for you, ensuring precision and quality.
Transposon Purification Service
We provide chromatographic purification and functionality testing for customer-supplied transposons or carrier plasmids amplified by us.
Free consultation available!
We are here to help you to design your experiments and find the right tools for your transposon mutagenesis application!
Contact us
Why Choose MuA for Transposon Mutagenesis
Transposable elements, or transposons, are DNA segments that can move within a genome, making them powerful tools for studying and manipulating genes across a wide range of organisms, including prokaryotes, plants, invertebrates, and vertebrates. Transposons are versatile because the DNA between their ends can include features like selectable markers, reporter genes, and regulatory elements. Widely used transposon systems include Sleeping Beauty, Tol2, PiggyBac, Tn5, and Mu, with factors like transposase-to-transposon ratio, DNA form, and cell type affecting their efficiency.
Why are Mu transposons ideal for genetic research?
The minimal Mu transposition system is characterized by high transposition frequency and low target-site selectivity, which makes it ideal for applications such as DNA sequencing, protein engineering, functional genome mapping, gene targeting, insertional mutagenesis, and SNP discovery.
Applications: From Bacteria to Mammalian Cells
Mu transposons have demonstrated efficient, species-independent gene delivery and insertional mutagenesis in a range of organisms, including bacteria, yeast, and mammalian cells. While PiggyBac, Sleeping Beauty, and Tol2 are widely used for gene delivery in mammalian cells, a notable advantage of Mu is its use of protein-DNA complexes for gene delivery. In contrast, transposon systems like PiggyBac, Sleeping Beauty, and Tol2 generally depend on plasmid-based methods for transposon delivery and integration.
👉 Read more about the current applications for Mu in vitro transposition technology
RECENT PUBLICATIONS
A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction
MuA Transposase
Protein engineering
Synthetic biology
Mu transpososome activity-profiling yields hyperactive MuA variants for highly efficient genetic and genome engineering
Hyperactive MuA Transposase
Cat-Mu Transposon
Genetic engineering
Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants and genome engineering
MuA Transposase
Haloferax volcanii
Insertion mutant library
Accessible and Insightful Scientific Learning Experiences Using the Microorganism Haloferax volcanii
MuA Transposase
Haloferax volcanii insertion mutant library
Archaea
Education
High school students