Domus Biotechnologies provides a wide selection of ready-to-use linear transposons for transposon mutagenesis, covering selectable markers for gram-negative bacteria, gram-positive bacteria and eukaryotic cells.
Schematic representation of Domus Biotechnologies transposons. Transposons contain different selectable marker genes between MuA transposase binding sites at both ends.
Transposons for bacteria
Cat-Mu Transposon (CamR)
Cat-Mu Transposon (CamR) contains marker gene cat, which confers resistance to chloramphenicol.
Kan-Mu Transposon (KanR)
Kan-Mu Transposon (KanR) contains marker gene kan, which confers resistance to kanamycin.
Transposons for eukaryotes
Puro-eGFP-Mu Transposon (PuroR-eGFP)
Puro-eGFP-Mu Transposon (PuroR-eGFP) contains a puromycin resistance-encoding gene under the SV40 promoter and eGFP-encoding gene under the human EF1α promoter.
Transposons for functional and structural studies of proteins
Cat-Mu Transposon (Stop)
Cat-Mu transposon (Stop) contains three translation stop signals close to each transposon end and marker gene cat, which confers resistance to chloramphenicol.