Domus Biotechnologies provides a wide selection of ready-to-use linear transposons for transposon mutagenesis, covering selectable markers for gram-negative bacteria, gram-positive bacteria and eukaryotic cells.
Transposons
Schematic representation of Domus Biotechnologies transposons. Transposons contain different selectable marker genes between MuA transposase binding sites at both ends.
Transposons for bacteria
Cat-Mu Transposon (CamR)
Cat-Mu Transposon (CamR) contains marker gene cat, which confers resistance to chloramphenicol.
Kan-Mu Transposon (KanR)
Kan-Mu Transposon (KanR) contains marker gene kan, which confers resistance to kanamycin.
Em-Mu Transposon (EmR)
Em-Mu Transposon (EmR) for grampositive bacteria. Contains marker gene ermB, encoding erythromycin resistance.
Km-Mu Transposon (KmR)
Km-Mu Transposon (KmR) for grampositive bacteria. Contains marker gene Km, which confers resistance to kanamycin.
Transposons for eukaryotes
Puro-eGFP-Mu Transposon (PuroR-eGFP)
Puro-eGFP-Mu Transposon (PuroR-eGFP) contains a puromycin resistance-encoding gene under the SV40 promoter and eGFP-encoding gene under the human EF1α promoter.
Kan/Neo-Mu Transposon (Kan/NeoR)
Kan/Neo-Mu Transposon (Kan/NeoR) contain the Kan/NeoR gene embedded between the SV40 promoter and HSV-TK terminator.
Transposons for functional and structural studies of proteins
Cat-Mu Transposon (Stop)
Cat-Mu transposon (Stop) contains three translation stop signals close to each transposon end and marker gene cat, which confers resistance to chloramphenicol.
Cat-Mu Transposon (NotI)
Cat-Mu Transposon (NotI) contains NotI restriction sites close to each transposon end and marker gene cat, which confers resistance to chloramphenicol.