Mini-Mu transposons can be used for generating templates for sequencing. In the reaction, the mini-Mu transposon is integrated in vitro into the target DNA to be sequenced. The reaction requires only one protein component, the MuA transposase, in addition to linear mini-Mu transposon DNA. The transposition reaction is highly efficient and produces relatively even distribution of integrations into the target DNA.
- Efficient and easy creation of Sanger sequencing templates
- Simple: no need for primer-walking or subcloning
- Efficient: generate thousands of sequencing templates from a single reaction
- Universal primers: a single set of primers can be used to sequence any clone, no need for custom primers