Kits and Applications
Transposon tools – accelerating gene delivery
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Transposon tools are commonly used to modify genes, genomes and proteins in both prokaryotic and eukaryotic organisms.
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Transposons can integrate efficiently and relatively randomly into target DNA.
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Bacteriophage Mu derived mini-Mu transposons are one of the most useful transposons due to their small size and well-characterized and simple in vitro transposition reaction.
Kits for Bacteria
Kit
Bacterial Genomic Integration Kit (Gram-negative, CamR)
Bacterial Genomic Integration Kit (Gram-negative, CamR) has been designed for efficient mutagenesis of all types of gram-negative bacterial strains.
Kit
Bacterial Genomic Integration Kit (Gram-negative, KanR)
Bacterial Genomic Integration Kit (Gram-negative, KanR) has been designed for efficient mutagenesis of all types of gram-negative bacterial strains.
Kit
Bacterial Genomic Integration Kit (Gram-positive, EmR)
Bacterial Genomic Integration Kit (Gram-positive, EmR) has been designed for efficient mutagenesis of gram-positive bacterial strains.
Kit
Bacterial Genomic Integration Kit (Gram-positive, KmR)
Bacterial Genomic Integration Kit (Gram-positive, KmR) has been designed for efficient mutagenesis of gram-positive bacterial strains.
Kits for Eukaryotes
Kit
Eukaryotic Genomic Integration Kit (PuroR-eGFP)
Eukaryotic Genomic Integration Kit (PuroR-eGFP) has been designed for efficient mutagenesis of mammalian cells.
Kit
Eukaryotic Genomic Integration Kit (Kan/NeoR)
Eukaryotic Genomic Integration Kit (Kan/NeoR) has been designed for efficient mutagenesis of mammalian cells.
Applications
Application
Generating templates for sequencing
Mini-Mu transposons can be used for generating templates for sequencing.
Application
Generating truncated proteins for functional studies
Mu transposition can be used to simultaneously generate a nested set of gene constructions encoding deletion variants of proteins.
Overview
Figure 1 (click to enlarge). Schematic of Mu DNA transposition reaction with precut mini-Mu transposon. The desired DNA sequence (i.e. selection marker, origin of replication, other DNA sequence) is flanked by 50 bp Mu Ends (R1 and R2 MuA binding sites). Target DNA can be genomic DNA or purified plasmid DNA, and transposition reaction can be accomplished in vivo or in vitro. Integration generates 5 bp target site duplication (TSD).
How MuA compares to Other Transposon Systems
The MuA transposase system offers a versatile solution for random genomic integration across
bacteria, yeast, and mammalian cells. Here’s how it compares to other popular systems:
| System | Host Range | Key Feature | Ready-to-Use Complexes |
|---|---|---|---|
| MuA transposase (our kits) | Bacteria, yeast, mammalian | Broad host range; random insertion; selectable markers | Yes |
| Sleeping Beauty | Mammalian | TA site targeting; gene therapy research | No |
| PiggyBac | Insect, mammalian | TTAA site preference; large cargo | No |
→ See full comparison of MuA, Sleeping Beauty, PiggyBac, and Tn5