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Kits and Applications

Transposon tools - accelerating gene delivery

  • Transposon tools are commonly used to modify genes, genomes and proteins in both prokaryotic and eukaryotic organisms.
  • Transposons can integrate efficiently and relatively randomly into target DNA.
  • Bacteriophage Mu derived mini-Mu transposons are one of the most useful transposons due to their small size and well-characterized and simple in vitro transposition reaction.

Genomic Integration Kit (CamR)

Genomic Integration Kit (CamR) has been designed for efficient mutagenesis of all types of gram-negative bacterial strains.

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Generating templates for sequencing

Mini-Mu transposons can be used for generating templates for sequencing.

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Generating truncated proteins for functional studies

Mu transposition can be used to simultaneously generate a nested set of gene constructions encoding deletion variants of proteins.

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Figure 1 (click to enlarge). Schematic of Mu DNA transposition reaction with precut mini-Mu transposon.  The desired DNA sequence (i.e. selection marker, origin of replication, other DNA sequence) is flanked by 50 bp Mu Ends (R1 and R2 MuA binding sites). Target DNA can be genomic DNA or purified plasmid DNA, and transposition reaction can be accomplished in vivo or in vitro. Integration generates 5 bp target site duplication (TSD).

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