Bacterial Genomic Integration Kits – Gram-Negative (KanR, CamR) & Gram-Positive (KmR, EmR)
Kit Overview
Our Genomic Integration Kits for bacteria enable efficient transposon mutagenesis in both Gram-negative and Gram-positive strains. Selectable markers include KanR, CamR, KmR, and EmR. Each kit comes with hyperactive v.3 MuA transpososome complexes, sequencing primers, and detailed protocols.
| Gram-Negative Kits | Gram-Positive Kits |
|---|---|
| Product ID: D070201 Bacterial Genomic Integration Kit (Gram-negative, KanR) – Kanamycin resistanceIncludes pre-assembled MuA transpososomes Price: 520 € (VAT 0%) Package Size: 10 reactions Buy Now |
Product ID: D070401 Bacterial Genomic Integration Kit (Gram-positive, KmR) – Kanamycin resistanceIncludes pre-assembled MuA transpososomes Price: 520 € (VAT 0% Package Size: 10 reactions Buy Now |
| Product ID: D070101 Bacterial Genomic Integration Kit (Gram-negative, CamR) – Chloramphenicol resistanceIncludes pre-assembled MuA transpososomes Price: 520 € (VAT 0%) Package Size: 10 reactions Buy Now |
Product ID: D070301 Bacterial Genomic Integration Kit (Gram-positive, EmR) – Erythromycin resistanceIncludes pre-assembled MuA transpososomes Price: 520 € (VAT 0%) Package Size: 10 reactions Buy Now |
Why choose Domus MuA transpososomes?
Domus MuA transpososomes provide a reliable and convenient solution for bacterial mutagenesis and genomic integration. Supplied ready to use, they eliminate the need to assemble transposition complexes, allowing you to focus on your research rather than reagent preparation.
Key benefits
✔ Ready-to-use MuA transpososomes
✔ Broad experimentally validated host range
✔ Superior efficiency with hypercative mutant MuA (v2 and v3 MuA transposases)
✔ Efficient random mutagenesis and genomic integration
✔ Single genomic insertion per cell
✔ Suitable for mutant library construction and genome engineering
✔ Expert scientific support tailored to your bacterial species and research goals
Broad host range
One of the key considerations when selecting a transposon mutagenesis system is host compatibility.
Will it work in my organism?
The answer is yes—for a remarkably wide range of organisms.
Mu transpososome technology has been successfully applied in numerous Gram-negative and Gram-positive bacterial species, making it an excellent choice for both model and non-model bacteria.
What is more, its versatility extends well beyond bacteria. Mu transpososomes have also been successfully used for efficient genomic integration in yeast (Saccharomyces cerevisiae), and mammalian cells, including human HeLa cells and human and mouse embryonic stem cells.
This exceptional breadth demonstrates the robustness of MuA transposition technology across diverse organisms and applications.
Another important advantage of Mu integration technology is that it generates a single genomic insertion per genome. This is highly desirable for genome engineering and particularly valuable for genome-wide insertion libraries and genetic screens, where single insertions simplify downstream analysis.
Explore our experimentally validated host range and see whether your organism has already been tested.
Switching from Tn5 to MuA
Researchers who previously relied on commercial Tn5 transposome kits are increasingly looking for reliable, ready-to-use alternatives backed by expert scientific support.
At Domus Biotechnologies, we have helped laboratories successfully transition to Domus MuA transpososomes for bacterial mutagenesis and genomic integration.
Whether you are continuing an established workflow or starting a new project, we’ll help you choose the most suitable MuA solution for your bacterial species and application.
If your laboratory has experience with commercial Tn5 transposome kits, transitioning to MuA is often straightforward.
Our scientists can help you:
- Select the most suitable MuA transpososome complex for your application
- Adapt your existing workflow
- Optimize transposition conditions for your bacterial species
- Design mutant library construction strategies
- Discuss genomic integration approaches
- Troubleshoot challenging applications
Our goal is not simply to provide a product—it is to help make your experiment successful.
General Workflow
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Electroporate transposition complexes into your bacterial strain
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Select integration clones on antibiotic selection plates
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Screen mutants using phenotypic assays, PCR, or sequencing
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Map insertion sites with included primers
Figure 1 (click to enlarge). Schematic of Mu DNA transposition reaction with precut mini-Mu transposon. The desired DNA sequence (i.e. selection marker, origin of replication, other DNA sequence) is flanked by 50 bp Mu Ends (R1 and R2 MuA binding sites). Target DNA can be genomic DNA or purified plasmid DNA, and transposition reaction can be accomplished in vivo or in vitro. Integration generates 5 bp target site duplication (TSD).
PhD Elsi Pulkkinen
Senior Scientist
Domus Biotechnologies
Talk with a scientist
Not sure which MuA Genomic Integration Kit is the best choice for your organism?
Tell us about your bacterial species, your research goals, and your experimental design—we’ll help you get started.
References
- Lamberg A, Nieminen S, Qiao M, Savilahti H (2002) Efficient insertion mutagenesis strategy for bacterial genomes involving electroporation of in vitro-assembled DNA transposition complexes of bacteriophage Mu. Appl Environ Microbiol 68:705-712
- Li, Y., Xia, H., Bai, F., Xu, H., Yang, L., Yao, H., Zhang, L., Zhang, X. et al. 2007. Identification of a new gene PA5017 involved in flagella-mediated motility, chemotaxis and biofilm formation in Pseudomonas aeruginosa. FEMS Microbiol Lett 272, 188–195.
- Pajunen MI, Pulliainen AT, Finne J, Savilahti H (2005) Generation of transposon insertion mutant libraries for Gram-positive bacteria by electroporation of phage Mu DNA transposition complexes. Microbiology 151:1209-1218
- Lanckriet A, Timbermont L, Happonen LJ, Pajunen MI, Pasmans F, Haesebrouck F, Ducatelle R, Savilahti H, Van Immerseel F (2009) Generation of single-copy transposon insertions in Clostridium perfringens by electroporation of phage Mu DNA transposition complexes. Applied and Environmental Microbiology 75:2638–2642.