Mu transposition can be used to simultaneously generate a nested set of gene constructions encoding deletion variants of proteins. C-terminal deletions can be produced using a mini-Mu transposon that carries translation stop signals at each transposon end. Similarly, N-terminal deletions can be generated using a transposon with appropriate restriction sites, which allows deletion of the 5’ -distal part of the gene.
- Create N- and C-terminal deletion variants of proteins for functional studies
- Generate a comprehensive repertoire of gene constructions in a single reaction
- Applicable to any protein-encoding gene cloned in an appropriate vector
- Fast and efficient way for producing a large number of protein deletion derivatives