High-efficiency transposition tools for in vitro library generation and in vivo genomic integration workflows.
Compare v2 and v3 | Contact a Scientist | Frequently asked questions
MuA transposition has been successfully applied in Gram-negative bacteria, Gram-positive bacteria and archaea, including E. coli, Salmonella, Pseudomonas, Staphylococcus, Listeria, Clostridium, Lactococcus, Corynebacterium and Haloferax volcanii. See the complete host range table and references.
⚡ Best for fast library generation
Best suited for researchers who need fast, efficient in vitro transposition for cloning, library construction, and engineered Mu-end applications.
🧬 Best for genomic integration workflows
Best suited for workflows where transpososomes are assembled in vitro and introduced into living cells for genomic integration.
Our customers have used v.2 MuA with modified Mu ends and achieved excellent results. In addition, our internal comparison data shows that v.2 MuA delivers substantially higher efficiency and a much faster workflow than wild-type MuA in modified-end in vitro reactions.
🔥 Highlight:
| Enzyme | Output | Reaction Time |
|---|---|---|
| v.2 MuA | 1.1 × 10⁶ CFU/µg transposon DNA | 10 min |
| Wild-type MuA | 9.8 × 10⁴ CFU/µg transposon DNA | 60 min |
v.2 MuA and v.3 MuA are distinct hyperactive mutants optimized for different environments. The distinction is not due to differences in Mu end sequences or cofactors. Instead, each variant has been selected to perform optimally under the specific reaction conditions required for in vitro or in vivo workflows.
For in vitro applications involving engineered or modified Mu ends, v.2 MuA significantly outperforms wild-type MuA in internal comparison data. This makes it an excellent choice for demanding in vitro workflows such as cloning, library construction, and experiments involving non-standard Mu ends.
Every transposition experiment is different. We’re happy to help you optimize your setup and get the best possible results.
Researchers evaluating v.2 MuA may request a complimentary wild-type MuA preparation for benchmarking.
PhD Elsi Pulkkinen
Senior Scientist
Domus Biotechnologies
Not sure which MuA variant fits your workflow? We’re happy to help you choose the right enzyme and optimize your transposition setup.
