Build Better Mutant Libraries Faster with Hyperactive MuA
High-efficiency transposition tools for in vitro library generation and in vivo genomic integration workflows.
Compare v2 and v3 | Contact a Scientist | Frequently asked questions
Designed for Demanding Transposition Workflows
- Hyperactive variants engineered for performance
- Proven in demanding in vitro and in vivo workflows
- Designed for modern microbial genetics and synthetic biology
Choose the Right MuA for Your Workflow
v.2 MuA - In vitro Manipulator
⚡ Best for fast library generation
Ideal for:
- Insertion libraries
- Cloning workflows
- Modified Mu ends
- Plasmids
- Linear DNA
- BAC / YAC applications
Key Benefits:
- Fast 10-minute reaction time
- ~10× higher output vs wild-type in internal comparison
- Excellent for demanding in vitro workflows
Description:
Best suited for researchers who need fast, efficient in vitro transposition for cloning, library construction, and engineered Mu-end applications.
v.3 MuA – In Vivo Integrator
🧬 Best for genomic integration workflows
Ideal for:
- Genomic integration assays
- Bacterial systems
- Eukaryotic systems
- Transpososome delivery workflows
Key Benefits:
- Optimized specifically for in vivo environments
- Stable transpososome-based workflow
- Strong choice for integration-focused projects
Description:
Best suited for workflows where transpososomes are assembled in vitro and introduced into living cells for genomic integration.
Not sure which MuA fits your workflow? We’re happy to help.
Performance with Modified Mu Ends
Our customers have used v.2 MuA with modified Mu ends and achieved excellent results. In addition, our internal comparison data shows that v.2 MuA delivers substantially higher efficiency and a much faster workflow than wild-type MuA in modified-end in vitro reactions.
🔥 Highlight:
- ~10× higher output
- 6× faster workflow
| Enzyme | Output | Reaction Time |
|---|---|---|
| v.2 MuA | 1.1 × 10⁶ CFU/µg transposon DNA | 10 min |
| Wild-type MuA | 9.8 × 10⁴ CFU/µg transposon DNA | 60 min |
Frequently Asked Questions
Why are different MuA variants recommended for in vitro vs in vivo applications?
v.2 MuA and v.3 MuA are distinct hyperactive mutants optimized for different environments. The distinction is not due to differences in Mu end sequences or cofactors. Instead, each variant has been selected to perform optimally under the specific reaction conditions required for in vitro or in vivo workflows.
Which MuA performs best with modified Mu ends?
For in vitro applications involving engineered or modified Mu ends, v.2 MuA significantly outperforms wild-type MuA in internal comparison data. This makes it an excellent choice for demanding in vitro workflows such as cloning, library construction, and experiments involving non-standard Mu ends.
We Support Your Workflow
Every transposition experiment is different. We’re happy to help you optimize your setup and get the best possible results.
We can assist with:
- Experimental design
- Reaction optimization
- Troubleshooting
- Product selection
Benchmarking Offer
Researchers evaluating v.2 MuA may request a complimentary wild-type MuA preparation for benchmarking.
PhD Elsi Pulkkinen
Senior Scientist
Domus Biotechnologies
Talk with a scientist
Not sure which MuA variant fits your workflow? We’re happy to help you choose the right enzyme and optimize your transposition setup.