MuA 15 bp Insertion Mutagenesis Kit

The MuA 15 bp Insertion Mutagenesis Kit is designed for generating random, defined 15 bp insertions in cloned target DNA. The kit enables the generation of diverse insertion libraries for protein engineering, functional mapping, regulatory DNA analysis, and viral genome mutagenesis.

The kit contains hyperactive v.2 MuA Transposase (in vitro Manipulator), Cat-Mu Transposon (NotI), Kan-Mu Transposon (NotI), 2X MuA Reaction Buffer, and Insertion Mapping Primer. In protein-coding regions, the resulting 15 bp insertions maintain the reading frame and introduce five additional amino acids.

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