| Genomic Integration Kits | MuA 15bp Insertion Mutagenesis Kit | Overview | Comparison with other systems | Contact Us
MuA Transposase Kits
Ready-to-use MuA transposase kits for genomic integration and insertion mutagenesis.
Domus Biotechnologies offers MuA-based kits for generating random genomic integrations in bacterial and eukaryotic cells, as well as defined 15 bp insertion libraries for functional analysis of proteins, regulatory DNA and viral genomes.
Genomic Integration Kits
Generate random, stable genomic insertions using ready-to-use MuA transpososome complexes.
Choose from kits optimized for Gram-negative bacteria, Gram-positive bacteria and eukaryotic cells.
Kit
Bacterial Genomic Integration Kit (Gram-negative, CamR)
Bacterial Genomic Integration Kit (Gram-negative, CamR) has been designed for efficient mutagenesis of all types of gram-negative bacterial strains.
Kit
Bacterial Genomic Integration Kit (Gram-negative, KanR)
Bacterial Genomic Integration Kit (Gram-negative, KanR) has been designed for efficient mutagenesis of all types of gram-negative bacterial strains.
Kit
Bacterial Genomic Integration Kit (Gram-positive, EmR)
Bacterial Genomic Integration Kit (Gram-positive, EmR) has been designed for efficient mutagenesis of gram-positive bacterial strains.
Kit
Bacterial Genomic Integration Kit (Gram-positive, KmR)
Bacterial Genomic Integration Kit (Gram-positive, KmR) has been designed for efficient mutagenesis of gram-positive bacterial strains.
Kit
Eukaryotic Genomic Integration Kit (PuroR-eGFP)
Eukaryotic Genomic Integration Kit (PuroR-eGFP) has been designed for efficient mutagenesis of mammalian cells.
Kit
Eukaryotic Genomic Integration Kit (Kan/NeoR)
Eukaryotic Genomic Integration Kit (Kan/NeoR) has been designed for efficient mutagenesis of mammalian cells.
MuA 15 bp Insertion Mutagenesis Kit
Kit
MuA 15 bp Insertion Mutagenesis Kit
Generate random 15 bp insertions for functional analysis of proteins and DNA.
Create diverse insertion libraries using v.2 MuA Transposase and selectable Cat-Mu (NotI) or Kan-Mu (NotI) transposons. Following transposon removal, a defined 15 bp insertion remains at each insertion site.
Applications include: protein engineering and functional mapping, promoter and regulatory DNA analysis, viral genome mutagenesis, and random NotI site insertion.
Applications
Application
Generating templates for sequencing
Mini-Mu transposons can be used for generating templates for sequencing.
Application
Generating truncated proteins for functional studies
Mu transposition can be used to simultaneously generate a nested set of gene constructions encoding deletion variants of proteins.
Overview
Figure 1 (click to enlarge). Schematic of Mu DNA transposition reaction with precut mini-Mu transposon. The desired DNA sequence (i.e. selection marker, origin of replication, other DNA sequence) is flanked by 50 bp Mu Ends (R1 and R2 MuA binding sites). Target DNA can be genomic DNA or purified plasmid DNA, and transposition reaction can be accomplished in vivo or in vitro. Integration generates 5 bp target site duplication (TSD).
How MuA compares to Other Transposon Systems
The MuA transposase system offers a versatile solution for random genomic integration across
bacteria, yeast, and mammalian cells. Here’s how it compares to other popular systems:
| System | Host Range | Key Feature | Ready-to-Use Complexes |
|---|---|---|---|
| MuA transposase (our kits) | Bacteria, yeast, mammalian | Broad host range; random insertion; selectable markers | Yes |
| Sleeping Beauty | Mammalian | TA site targeting; gene therapy research | No |
| PiggyBac | Insect, mammalian | TTAA site preference; large cargo | No |
→ See full comparison of MuA, Sleeping Beauty, PiggyBac, and Tn5