Eukaryotic Genomic Integration Kits

Kit Description

Our Eukaryotic Genomic Integration Kits harness the power of the V.3 MuA transposase to achieve efficient and reliable genomic integration in mammalian cells.

Kit Name	Product Code	Selectable Marker	Promoter / Terminator	Reporter	Applications	Package Size	Price
Eukaryotic Genomic Integration Kit (PuroR-eGFP)	D070501	Puromycin resistance gene (PuroR)	SV40 promoter (PuroR); EF1α promoter (eGFP)	eGFP	In vivo transposon mutagenesis in mammalian cells	10 reactions	520 €	Request a Quote
Eukaryotic Genomic Integration Kit (Kan/NeoR)	D070601	Kan/NeoR gene	SV40 promoter + HSV-TK terminator	–	In vivo transposon mutagenesis in mammalian cells; dual selection in E. coli	10 reactions	520 €	Request a Quote

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Applications

In vivo transposon mutagenesis in mammalian cells
Stable reporter gene integration for tracking expression and lineage tracing
Antibiotic selection of engineered cells (puromycin, kanamycin/neomycin)
Dual host utility with Kan/NeoR transposon (works in both mammalian cells and E. coli)
Gene function studies through random insertional mutagenesis
Cell line engineering for research or preclinical development

Benefits

✔ Simple – Kit contains ready-to-use Mu DNA transposition complexes
✔ Efficient integration – MuA transposase mediates high-frequency, random genomic insertions
✔ Broad applicability – validated in both yeast and mammalian systems (Paatero et al., 2008)
✔ Selectable markers – puromycin or kanamycin/neomycin resistance for robust selection
✔ Reporter readout – eGFP for visual confirmation of successful integration
✔ Flexible host range – Kan/NeoR option supports selection in mammalian cells and E. coli
✔ Reproducible workflows – based on a proven, well-characterized transposase system

General Workflow

Delivery into mammalian cells
Introduce the ready-to-use transposition complexes via electroporation.
Genomic integration
MuA catalyzes random insertion of the transposon into host genomic DNA.
Selection & confirmation
Apply appropriate selection (puromycin or kanamycin/neomycin).
Confirm integration via GFP expression (Puro-eGFP) or molecular analysis (PCR, sequencing).
Downstream applications
Use engineered cells for mutagenesis studies, gene discovery, or functional assays.

Figure 1 (click to enlarge). Schematic of Mu DNA transposition reaction with precut mini-Mu transposon. The desired DNA sequence (i.e. selection marker, origin of replication, other DNA sequence) is flanked by 50 bp Mu Ends (R1 and R2 MuA binding sites). Target DNA can be genomic DNA or purified plasmid DNA, and transposition reaction can be accomplished in vivo or in vitro. Integration generates 5 bp target site duplication (TSD).

References

Paatero AO, Turakainen H, Happonen LJ, Olsson C, Palomäki T, Pajunen MI, Meng X, Otonkoski T, Tuuri T, Berry C, Malani N, Frilander MJ, Bushman FD, Savilahti H (2008) Bacteriophage Mu integration in yeast and mammalian genomes. Nucleic Acids Res 36:e148
Rasila,T.S., Pulkkinen,E., Kiljunen,S., Haapa-Paananen,S., Pajunen,M.I., Salminen,A., Paulin,L., Vihinen,M., Rice,P.A. and Savilahti,H. (2018) Mu transpososome activity-profiling yields hyperactive MuA variants for highly efficient genetic and genome engineering. Nucleic Acids Res., 46, 4649–4661.