We are here to help you find the right tools for your transposon mutagenesis application, whether it is gene delivery, insertional mutagenesis, knocking out genes, functional and structural studies of proteins, or something else. There are numerous ways how transposon technology can be used in genetic research and the number of applications keeps growing!
We Offer Hyperactive MuA Transposases, Transposons, Kits and Insertion Mutant Libraries
Domus Biotechnologies provides MuA transposases for in vitro and in vivo transposon mutagenesis.
Our highly efficient MuA variants (v.2 MuA Transposase and v.3 MuA Transposase) are excellent partners for demanding molecular biology applications, both for bacteria and eukaryotic cells.
Our MuA transposases can be used to insert any artificial transposon with MuA binding sites at both ends, into virtually any target DNA in random sites.
Domus Biotechnologies provides a wide selection of ready-to-use linear transposons for transposon mutagenesis, covering selectable markers for gram-negative bacteria, gram-positive bacteria and eukaryotic cells.
Genomic Integration Kit
Genomic Integration Kit (CamR) has been designed for efficient mutagenesis of all types of gram-negative bacterial strains.
Genomic Integration Kit is easy to use, simply electroporate Mu DNA Transposition Complexes (CamR) (transpososome) into your bacterial strain of interest. To select the clones with transposon integration, grow bacterial clones on selective (chloramphenicol) media.
Electroporation of Mu DNA transposition complexes has been used successfully with many bacterial species, including gram-negatives, such as coli, Salmonella enterica, Erwinia carotovora, Yersinia enterocolitica, Erwinia carotovora, Pseudomonas aeruginosa.
insertion mutant library
Transposons are powerful tools for the generation of insertion mutant libraries in various organisms, in which each gene is tagged by an insertion.
Our Haloferax volcanii library contains an exhaustive pool of mutants, in which each clone contains one genomic insertion of a transposon in a random position and with a full-genome coverage.
Transposable elements, or transposons, are DNA segments that have the ability to move from one location on the genome to another. They have been widely used for investigating and manipulating genes and genomes of prokaryotes, plants, invertebrates, and recently also vertebrates. Transposons are extremely suitable for several different purposes, as the DNA between the transposon ends can be of any origin and can include useful features such as selectable markers, unique primer binding sites, reporter functions (such as the gene for β-galactosidase), plasmid origins of replication, and controlling elements (such as regulated promoters).
What makes Mu transposons great for genetic research?
The minimal Mu transposition system displays high transposition frequency and relatively low target-site selectivity, and these features make it ideal for a variety of applications. Mu transposons have been used for DNA sequencing, protein engineering for structure/function studies, genome-wide functional mapping of virus genomes, construction of gene targeting vectors, insertional mutagenesis of archaea, and SNP discovery. Mu transposons have also been used for highly efficient, species-non-specific gene delivery and insertional mutagenesis, as demonstrated with a variety of Gram-negative and Gram-positive bacteria, yeast, and mammalian cells. Read more about the current applications for Mu in vitro transposition technology here.
A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction
Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants and genome engineering
Insertion mutant library
Accessible and Insightful Scientific Learning Experiences Using the Microorganism Haloferax volcanii
Haloferax volcanii insertion mutant library
High school students